Knife Edge Scanning Microscope (KESM)


3Scan images entire sample volumes of over 100 cm3 at cellular level resolution, combining elements of non-invasive radiology and light microscopy. Our KESM can process up to 3,600 slices per hour (e.g. a whole mouse brain) of any tissue sample at submicron resolution (a voxel size of 0.6 um x 0.7 um x 1.0 um).

To handle data outputs of up to a terabyte per cm3, 3Scan has built customized processing and analysis software. 3Scan offers data processing software to model 3D tissue reconstructions, provide interactive image views, and apply quantitative analytics

The unprecedented volume of high-resolution 3D data generated by the KESM opens up a new realm of possibilities for meso-and micro-scale insights.

Applications include:

  • Large volume 2D fly-throughs (~200-800 sections per cm3)
  • 3D visualizations and models of various tissues (i.e. tumor morphology)
  • Co-registration of features from non-invasive radiology and light microscopy
  • Automated quantification and analysis of specific tissue structures (e.g. microvasculature)

3Scan Founder/CEO, Todd Huffman – Understand systems biology from the inside out

3Scan Founder/COO, Megan Kliman – How we look at tissue samples doesn’t tell the whole story


Have a project you could use 3Scan for?

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